Effective micropropagation of kale (Brassica oleracea convar. Acephala var. sabellica): one of the most important representatives of cruciferous crops

Abstract Kale ( Brassica oleracea convar. acephala var. sabellica ) is one of the oldest vegetable varieties with highest nutritional value and stress resistance. This work aimed to establish an efficient kale micropropagation protocol. Shoot tips were used as explants during axillary shoots multiplication. The most effective cytokinin (6 10 per explant after 1st 2nd passage, respectively) was 2.5 mg dm −3 6-benzylaminopurine. Rooting on MS medium supplemented 1.0 indole-3-acetic acid (IAA; 95% rooted shoots). survival rate acclimatization ex vitro conditions occurred when plantlets planted in a soil perlite mixture. No changes DNA content detected using flow cytometry. paper additionally emphasizes problems associated abnormal development some multiplied Brassicaceae shoots. Nevertheless, results confirmed that successful without any phenotypic aberration.